LH Elisa 96T

Introduction LH Elisa 96T

LH Elisa 96T

Application of LH Elisa 96T kit

The Kit ELISA LH of Arghvan Teb Kavian Company is designed for laboratory quantitative measurement of luteal hormone (LH) in serum or plasma. This kit is supplied in a package of 96 tests by Arghvan Teb Kavian Company. Luteal hormone or LH together with follicle stimulating hormone (FSH) is secreted by the gonadotropin cells of the anterior pituitary and synergistically they are responsible for regulating and stimulating the function of the sex glands (ovaries and testicles).

This hormone, like FSH, hCG, and TSH, is a two-chain glycoprotein hormone consisting of a 92 amino acid long α chain and a 121 amino acid long β chain, which are non-covalently linked to each other. In women, gonadotropins in the hypothalamus circuit, the pituitary gland of the gonads are responsible for controlling the menstrual cycle. LH and FSH are secreted in waves from the gonadotrope cells of the pituitary gland and enter the blood.

In the blood, gonadotropins stimulate the growth and maturation of follicles and the production of estrogens and progesterones. The maximum amount of LH is observed in the middle of the menstrual cycle, which induces the release of the egg and the formation of the corpus luteum, the main secreted hormone of which is progesterone. In men, LH stimulates testosterone production from Leydig cells.

Since these two hormones are secreted in waves, a single measurement may lead to false results. A solution to overcome this problem is to obtain two to three samples from the patient with an interval of 20 to 30 minutes and then accumulate the sera. It is equal to the measurement of the hormone in the pooled sample.

Determining the amount of LH helps the doctor in finding the cause of hypothalamus-pituitary-endocrine dysfunction. The measurement of LH and FSH is used in cases such as congenital diseases with chromosomal abnormalities (for example, Turner's syndrome), polycystic ovary (PCO), discovering the causes of menopause, menopausal symptoms, and Leydig cell failure.

Measurement of LH and FSH is very useful in differentiating primary gonadal insufficiency from secondary insufficiency. In primary insufficiency, such as that seen in PCO or menopause, the amount of these two hormones increases significantly, while in secondary insufficiency caused by pituitary dysfunction, the amount of these two hormones decreases. Also, a sudden increase in the level of LH is a sign of the imminent release of the egg from the follicles, which can indicate a high probability of pregnancy.

Technical Specifications LH Elisa 96T

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