Free T3 Elisa 192T

Introduction Free T3 Elisa 192T

Free T3 Elisa 192T

Application of Free T3 Elisa 192T kit

Kit ELISA 3T Free of Arghvan Teb Kavian Company is designed for quantitative measurement of free triiodothyronine hormone or 3T Free in serum or plasma. This kit is supplied in a package of 192 tests by Arghvan Teb Kavian. Two hormones thyroxine (T4) and triiodothyronine (T3) are produced by the thyroid gland and enter the blood. Only 20% of T3 is made directly by the thyroid, and 80% is made by the 5-iodinase enzyme in the tissue from the removal of an iodine atom from thyroxine. Because T3 has a shorter half-life compared to T4 (2 days compared to 7 days).

Therefore, it seems that thyroxine acts mainly as a storage source and precursor of T3 hormone, which is the most biologically active form of thyroid hormones. Like T4, the majority of T3 present in the blood is also transferred bound to TBG, prealbumin and albumin proteins, but the affinity of TBG to T3 is lower compared to T4, so compared to thyroxine, there is a larger fraction of T3 in free form. (0.3% of T3 compared to 0.02% of T4). Due to the daily production of three times more T4 than T3, the absolute amount of free T4 exceeds free T3. The main driver of thyroid hormone production is thyrotropin hormone or TSH secreted from the pituitary gland, which is under the control of thyrotropin-releasing hormone (TRH) produced by the hypothalamus, both of which are under the control of T3 and T4.

Thyroid hormones enhance many intracellular actions and promote cell growth and differentiation. Thyroid hormones increase oxygen consumption and ATP production, mitochondrial metabolism, synthesis and metabolism of carbohydrates, synthesis and breakdown of cholesterol and triglycerides (for example, through the regulation of LDL receptor expression in the liver), tissue sensitivity to catecholamines. . It also increases heart rate and contractility of heart muscles. In addition to the mentioned cases, thyroid hormones play an important role in regulating calcium and phosphorus metabolism, growth and development of fetuses and children.

The amount of T3 hormone, like T4, increases in hyperthyroidism and decreases in hypothyroidism, which may be associated with a decrease or increase in TSH, respectively. Factors such as pregnancy or the use of oral contraceptive pills and acute inflammatory liver diseases that increase the concentration of thyroid hormone binding proteins can increase the amount of total thyroid hormones in the serum, while the amount of free hormones in these people It is natural.

In these people, TSH is usually in the reference range. Since only the free form of the hormone is biologically active, measuring the free hormone, especially in the presence of factors that cause a change in the amount or tendency of binding proteins to thyroid hormones, can better find the reason for the discrepancy between the results of the total hormone and the amount. Serum TSH helps. Some drugs such as amidarone or propranolol inhibit the iodinase enzyme and reduce the environmental conversion of T4 to T3. In these people, despite the increase in T4, we see a decrease in T3 and normal or high TSH. In 95% of cases, toxic thyroid (total or free) and TSH are sufficient for diagnosis, but in 2 to 5% of cases, an increase is observed only in T3 (T3 toxicosis or T3) (Kit ELISA 3T Free company)

Arghvan Teb Kavian is designed for quantitative measurement of free triiodothyronine or 3T Free hormone in serum or plasma.

This kit is supplied in a package of 192 tests by Arghvan Teb Kavian. Two hormones thyroxine (T4) and triiodothyronine (T3) are produced by the thyroid gland and enter the blood. Only 20% of T3 is made directly by the thyroid, and 80% is made by the 5-iodinase enzyme in the tissue from the removal of an iodine atom from thyroxine. Because T3 has a shorter half-life compared to T4 (2 days compared to 7 days). Therefore, it seems that thyroxine acts mainly as a storage source and precursor of T3 hormone, which is the most biologically active form of thyroid hormones.

Like T4, the majority of T3 present in the blood is also transferred bound to TBG, prealbumin and albumin proteins, but the affinity of TBG to T3 is lower compared to T4, so compared to thyroxine, there is a larger fraction of T3 in free form. (0.3% of T3 compared to 0.02% of T4). Due to the daily production of three times more T4 than T3, the absolute amount of free T4 exceeds free T3. The main driver of thyroid hormone production is thyrotropin hormone or TSH secreted from the pituitary gland, which is under the control of thyrotropin-releasing hormone (TRH) produced by the hypothalamus, both of which are under the control of T3 and T4. Thyroid hormones enhance many intracellular actions and promote cell growth and differentiation.

Thyroid hormones increase oxygen consumption and ATP production, mitochondrial metabolism, synthesis and metabolism of carbohydrates, synthesis and breakdown of cholesterol and triglycerides (for example, through the regulation of LDL receptor expression in the liver), tissue sensitivity to catecholamines. . It also increases heart rate and contractility of heart muscles. In addition to the mentioned cases, thyroid hormones play an important role in regulating calcium and phosphorus metabolism, growth and development of fetuses and children. The amount of T3 hormone, like T4, increases in hyperthyroidism and decreases in hypothyroidism, which may be associated with a decrease or increase in TSH, respectively.

Factors such as pregnancy or the use of oral contraceptive pills and acute inflammatory liver diseases that increase the concentration of thyroid hormone binding proteins can increase the amount of total thyroid hormones in the serum, while the amount of free hormones in these people It is natural. In these people, TSH is usually in the reference range. Since only the free form of the hormone is biologically active, measuring the free hormone, especially in the presence of factors that cause a change in the amount or tendency of binding proteins to thyroid hormones, can better find the reason for the discrepancy between the results of the total hormone and the amount. Serum TSH helps.

Some drugs such as amidarone or propranolol inhibit the iodinase enzyme and reduce the environmental conversion of T4 to T3. In these people, despite the increase in T4, we see a decrease in T3 and normal or high TSH. In 95% of cases, toxic thyroid (total or free) and TSH are sufficient for diagnosis, but in 2-5% of cases, an increase is observed only in T3 (T3 toxicosis or T3).

Technical Specifications Free T3 Elisa 192T

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